dear SnowDeer,
The first step to see if bigger crystals can be grown is to use bigger
protein drops and vary
the precipitant/protein ratio at the beginning of the vapour diffusion
experiment.
You can work out for yourself why this
should give you all the informations that you need in subsequent
experiments.
Enrico.
On Mon, 05 Sep 2011 10:06:22 +0200, SnowDeer <huanxu...@gmail.com> wrote:
Dear All:
Recently I am working on a protein which can already grow nice
pyramid-like
crystals after the condition was optimized, while the crystals are too
small
to be picked up. The crystals grew quite fast and densely, so I tried to
put
100ul paraffin oil inside the 600ul reservoir solution or put the plate
under 16 degree to slow down the evaporation, while the crystals were
still
the same. I also tried macro or micro seeding with or without the
paraffin
oil. Macroseeding would give a larger crystal (not very nice) with many
small crystals in the drop even I washed the seeds carefully. For
microseeding, the same small crystals grew.
I don't have many experiences in crystallography, so I have no idea how
to
make it grow bigger...
Any suggestion is most welcome.
Thanks.
SnowDeer
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
