>"vary the precipitant/protein ratio" Agree with Enrico. Reducing the concentration of your protein sample to make it form less nuclei, then it might grow bigger.
Best R, tiantian On Mon, Sep 5, 2011 at 7:48 PM, Enrico Stura <est...@cea.fr> wrote: > dear SnowDeer, > > The first step to see if bigger crystals can be grown is to use bigger > protein drops and vary > the precipitant/protein ratio at the beginning of the vapour diffusion > experiment. > You can work out for yourself why this > should give you all the informations that you need in subsequent > experiments. > > Enrico. > > > > On Mon, 05 Sep 2011 10:06:22 +0200, SnowDeer <huanxu...@gmail.com> wrote: > > Dear All: >> >> Recently I am working on a protein which can already grow nice >> pyramid-like >> crystals after the condition was optimized, while the crystals are too >> small >> to be picked up. The crystals grew quite fast and densely, so I tried to >> put >> 100ul paraffin oil inside the 600ul reservoir solution or put the plate >> under 16 degree to slow down the evaporation, while the crystals were >> still >> the same. I also tried macro or micro seeding with or without the paraffin >> oil. Macroseeding would give a larger crystal (not very nice) with many >> small crystals in the drop even I washed the seeds carefully. For >> microseeding, the same small crystals grew. >> >> I don't have many experiences in crystallography, so I have no idea how to >> make it grow bigger... >> >> Any suggestion is most welcome. >> >> Thanks. >> SnowDeer >> > > > -- > Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office > Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab > LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE > http://www-dsv.cea.fr/en/**institutes/institute-of-** > biology-and-technology-saclay-**ibitec-s/unites-de-recherche/** > department-of-molecular-**engineering-of-proteins-** > simopro/molecular-toxinology-**and-biotechnology-laboratory-** > ltmb/crystallogenesis-e.-stura<http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura> > http://www.chem.gla.ac.uk/**protein/mirror/stura/index2.**html<http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html> > e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71 > -- Shanghai Institute of Materia Medica, Chinese Academy of Sciences Address: Room 101, 646 Songtao Road, Zhangjiang Hi-Tech Park, Shanghai, 201203
