Thanks a lot for your suggestions. Would it be worth while to try and remove glycerol at the last step which is gel filtration and then set trays in my case? I have never tried without glycerol. reg. Anita
On Fri, Sep 9, 2011 at 7:54 PM, Enrico Stura <est...@cea.fr> wrote: > Anita, > > see the message I posted yesterday on the CCP4bb: > http://www.mail-archive.com/**ccp4bb@jiscmail.ac.uk/**msg22638.html<http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg22638.html> > > or google "stura glycerol" > Re: [ccp4bb] How to help crystal grow bigger > > In your case the sentence you need is: > "You need to have a higher (double) glycerol concentration in the reservoir > else you will risk finding that your drops will get biggger and not smaller. > This note of caution applies to vapour diffusion set ups as equilibration > can be tricky in such context: Vera,L., Czarny, B., Georgiadis, D., Dive, > V., Stura, E.A. (2011) Practical Use of Glycerol in Protein Crystallization. > Cryst. Growth & Des. 11 :2755–2762. " > > http://pubs.acs.org/doi/abs/**10.1021/cg101364m<http://pubs.acs.org/doi/abs/10.1021/cg101364m> > > Enrico. > > > > On Fri, 09 Sep 2011 13:22:53 +0200, anita p <crystals...@gmail.com> wrote: > > Dear Crystallographers, >> I have set hanging drop trays with 2ul of protein and 2 ul of resorvior >> solution. I have seen in some cases the drops are swelling. My protein >> buffer has 15% glycerol in it. >> This is happening mainly when I have peg 400 or peg MME or MPD or >> Jeffamine >> in the buffer condition. >> Could any one suggest a remedy for this. >> >> with regards >> Anita >> > > > -- > Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office > Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab > LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE > http://www-dsv.cea.fr/en/**institutes/institute-of-** > biology-and-technology-saclay-**ibitec-s/unites-de-recherche/** > department-of-molecular-**engineering-of-proteins-** > simopro/molecular-toxinology-**and-biotechnology-laboratory-** > ltmb/crystallogenesis-e.-stura<http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-saclay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-proteins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallogenesis-e.-stura> > http://www.chem.gla.ac.uk/**protein/mirror/stura/index2.**html<http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html> > e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71 >
