Hi, Before coming to my current lab, I had used a Prime for a couple of years. Did the job, no complaints. Since being exposed to the Akta Express though, I would have to say that this is a great machine for purification of multiple constructs at the same(ish) time and I would really, really miss it if I had to go back to Prime/Purifier. Also using automatic runs combining several columns is also a great bonus. I would like to sort-of hijack/add to this thread (again) and ask of alternatives to this machine specifically, and people's experiences with it please?
cheers charlie Dr. Charles Allerston Genome Integrity & Repair Structural Genomics Consortium Nuffield Department of Medicine Old Road Campus University of Oxford OX3 7DQ http://www.thesgc.org/scientists/groups/oxford/genome-integrity-and-repair From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Paul Smith Sent: 13 October 2011 02:19 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Akta Prime / FPLC Options / Off Topic Michael, Unfortunately, I actually don't know who serves these machines apart from GE. Because you brought up the subject of GE equipment and service, I thought I would ask the community about the best options for routine crystallographic scale FPLC. In my opinion, following the takeover of Pharmacia by GE the the price of GE machines, replacement parts, and service has skyrocketed and GE service reps seem determined to squeeze and extort every dollar they can. Personally, I'd love to never do business with GE again. However, in some ways, they are the only game in town. GE is the de facto standard for our line of work and the Akta line are very good machines. However, GE's consistent price gouging and outright crooked service practices encourage me look elsewhere. I've used systems from AP-biotech (junk) and have heard some good things about Bio-rad. What does the community at large think? Are there other good options? Does anyone have some spare millions and manufacturing connections in India/China to consider starting a competing company? Sorry to hijack your thread Michael. Let me know what you find out. The less money I send to GE the better. --Paul ________________________________ From: Michael Colaneri <colane...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, October 12, 2011 2:28 PM Subject: [ccp4bb] Akta Prime Dear all, We have an AktaPrime and GE Lifesciences stop servicing these instruments because they are getting old. Does anyone know of a third party company that gives contracts to maintain these instruments? Thank you. Mike Colaneri
