Attached is a picture of making the bookend setup - step b. shows the razor levering up the flap from the plastic coverslip - be careful not to cut right through (I have done that and the drop dries extra quick in such a case) also be careful to apply the protein/well droplet as shown with to the shiny side of the lifted flap. (Apologies for the attachment).
hope your crystals go well. Martyn ________________________________ From: Nian Huang <huangn...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, 15 November 2011, 17:44 Subject: Re: [ccp4bb] [off topic] Control of crystals' direction and position in the drop. Thank you guys. I basically tried almost everything that I can find in the hampton catalogue and in this bulletin, seeding, hanging, sitting, sandwitch drops (which made things worse), temperature, gel, oil batch, and additive screens. Manipulating the crystal with fiber increases the chance to make it grow twin. The "book end" coverslip sounds a fantasic idea. It might be just going to work. Best, Nian
<<attachment: bookend.jpg>>
