Hi, I am routinely using the gryphon robot from Art Robbins.This instrument can dispense 0.02microlitre at minimum. Your drops can dry out if you use such low volumes you have to be really fast. You can set up 0.2 to 0.1 micro litre drops using this for 96 well plates however the instrument needs to be calibrated well sometimes with low volume drop the robot dispenses the protein at one corner of the well and the precipitant at another end so eventually the outcome is the mixing of the precipitant with your protein is not happening. However with 0.2micro litre drop or higher this is less of a problem. HTH, shya
On Fri, Nov 18, 2011 at 9:34 AM, xaravich ivan <xaravich.i...@gmail.com>wrote: > Hi, > I apologize in advance as it is not a ccp4 related question, but over the > years, CCP4bb is synonymous with protein crystallographers virtual > university, at least for me. > > Ok, now I do not have an easy access to crystallization robot, so I was > hoping if someone here have ever used the 96 well plates for manually > setting drops with much lower solution/sample volumes (0.1-0.2micro litres). > > I heard about a clicker syringe that can be used to manually add lesser > volumes but I am not sure how to go about it. > Please let me know if you are aware of or are routinely setting 96 well > trays with much lower volumes of crystallization solutions as well as > samples, manually. > > thanks in advance, > ivan >
