Hello Ivan,
You can dispense 0.1-0.2 uL drops with the PB-100 repeating
dispenser from Hamilton and a 10 uL glass syringe. But it's rather
clumsy and hard on the hands. I don't recommend using it with 96 well
plates. It's too slow to do the whole plate at one time without your
drops drying up.
Ho
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
holeung...@hawaii.edu
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From: xaravich ivan <xaravich.i...@gmail.com>
Subject: Manually setting 96 wells plates with lower volume samples!
Hi,
I apologize in advance as it is not a ccp4 related question, but over the
years, CCP4bb is synonymous with protein crystallographers virtual
university, at least for me.
Ok, now I do not have an easy access to crystallization robot, so I was
hoping if someone here have ever used the 96 well plates for manually
setting drops with much lower solution/sample volumes (0.1-0.2micro litres).
I heard about a clicker syringe that can be used to manually add lesser
volumes but I am not sure how to go about it.
Please let me know if you are aware of or are routinely setting 96 well
trays with much lower volumes of crystallization solutions as well as
samples, manually.
thanks in advance,
ivan
