Hi there,
Thanks everyone who answered the post entitled "artificial tetramer" .
This is a resume of things I have done to build artificial tetramers.
The following are the steps I have used:
1) Using your helix as model, run Amore scenario "get origin shifted
model" with option "Reorient Trail Model" checked (From Eleonor
Dodson): this will give your helix perfectly oriented into a P1 cell;
2) With pdbset, translate your helix by the desired distance along X and
apply the desired symmetry to Z (P4 in my case): this will give your
template;
3) Rename the chain´s template and write a simple script combining
"lskab" and "pdb_merge" in a FOR loop to superimpose to and merge the
mobile pdb with your template one chain after another.
In principle I was thinking about get a arbitrary point into the cell,
pass a vector through it and apply the desired symmetry. However, unless
XFIT, I don't know which program else has these capabilities. So the
steps described here in are not of general application, but has solved
my problem.
Best wishes and Marry Xmas,
Fred
