If you haven't done so already, I would screen buffer conditions (pH, salt concentration, glycerol, strongly reducing conditions, ligands, detergents) by DLS to see if you can reduce aggregation. You might get lucky by setting up crystallization plates, but chances are you won't get very useful information from them, especially if your aggregated protein is soluble.
There are fluorescent dyes sensitive to aggregation state such as ANS (anilinonaphthalene-8-sulfonate) or Nile Red. I have not used them myself and would like to hear if others have found them useful for screening buffer conditions. Ho Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry h...@hawaii.edu
