Phusion requires that the primers are phosphorylated for mutagensis to work, unlike Pfu. If you cannot phosphorylate them use Pfu as recommended by Charlotte
Dan
- [ccp4bb] about point mutation Arun Kumar
- Re: [ccp4bb] about point mutation Tim Gruene
- Re: [ccp4bb] about point mutation Charlotte Hodson
- Re: [ccp4bb] about point mutation Xiaodi Yu
- [ccp4bb] coot with probe and reduce Rajesh kumar
- Re: [ccp4bb] coot with probe and reduce Bernhard C. Lohkamp
- Re: [ccp4bb] coot with probe and redu... Rajesh kumar
- Re: [ccp4bb] about point mutation D Bonsor
- Re: [ccp4bb] about point mutation Dima Klenchin
