-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Theresa,
I was not aware you need DTT for TEV protease activity. People do on-column digestion, and as far as I remember, a Ni-column would turn really uglily brown of you used DTT on those columns. Have you tried to leave out DTT and the cleavage did not work? Cheers, Tim On 04/16/12 09:31, Theresa Hsu wrote: > Dear all > > I want to digest a tagged protein with TEV protease, it has > disulfide bridges. Is there any way of doing cleavage without DTT? > > Thank you. > > Theresa > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFPi+H1UxlJ7aRr7hoRAjguAKCxEGcV2i6r8C31HBPIinJUI+PaogCgoBNb TrcS2lfQ753D4LmZ3tGNYok= =adb3 -----END PGP SIGNATURE-----
