I have used 5 mM beta-Mercaptoethanol, which is a weaker reducing agent than DTT, and that keeps TEV happy as well.
Cheers Florian Am 16.04.2012 um 09:31 schrieb Theresa Hsu: > Dear all > > I want to digest a tagged protein with TEV protease, it has disulfide > bridges. Is there any way of doing cleavage without DTT? > > Thank you. > > Theresa ------------------------------------------------------------- Dr. Florian Brückner Biomolecular Research Laboratory OFLG/102 Paul Scherrer Institut CH-5232 Villigen PSI Switzerland Tel.: +41-(0)56-310-2332 Email: florian.brueck...@psi.ch
