Just in case you are blocking with BSA, how about, e. coli beta-galactosidase (114 KDa) ?. It's commercially available but you probably have cells already making it in the lab
On 26 April 2012 19:29, Peter Hsu <hsuu...@u.washington.edu> wrote: > Hi all, > > Sorry for the very off topic problem. I'm looking for a loading control > for my westerns (doing in vitro assays). Due to separation/antibody > specificity issues, I need super good separation between 70 and 100 kDa, > oftentimes running those markers all the way to the bottom of the gel. If > I'm careful, I can usually keep the 55kDa marker still on the gel, but my > loading control is smaller and runs off. Does anyone know of a commercially > available protein that's relatively cheap, has an antibody against it, and > is either between 55-70kDa, or >100kDa to add into my experiments so I can > have a loading control? > > Thanks and sorry again for the off topic question. > > All the best, > > Peter >
