Hi All, I would like to have your expert advice on crystals. I am using detergents as 5% (w/v) of DDM 0.4ul in a 4ul (protein + condition + detergent). The precipitant is 28% peg 20K After 1 day I am able to see little plates of irregular shape .
I am able to see some needles if I change into MEGA-10. I am able to see some hexagonal 3D crystals when I change the precipitant to Ammonium sulphate and the detergent to Anapoe-C10E6 Is there a possibility to check whether this crystals are protein or of detergent, even before shooting it ? Does the control drop test help ?? awaiting for feedback Anita
