I calculated threefold averaged omit maps in coot. These maps look nice and clean, but I am having trouble making sense of the displayed sigma levels or e/A3 values. When I display the unaveraged and averaged maps at a similar density level for the protein the unaveraged map is at 0.024 e/A3 and 2.7 sigma, while the averaged map is displayed at 0.0016e/A3 and 7.6 sigma. I read the previous discussion about this issue where it was recommended to rely on the e/A3 values for comparison, but even that doesn't seem to work in this case. Any suggestions?
Thanks Ursula -- Ursula Schulze-Gahmen, Ph.D. Assistant Researcher UC Berkeley, QB3 356 Stanley Hall #3220 Berkeley, CA 94720-3220
