Have you tried multiple rounds of micro-seeding? ------------------------------------------------------------------------------- Greg Costakes PhD Candidate Department of Structural Biology Purdue University Hockmeyer Hall, Room 320 240 S. Martin Jischke Drive, West Lafayette, IN 47907
-------------------------------------------------------------------------------- ** Hard work often pays of in time, but Procrastination always pays off now ** ----- Original Message ----- From: "Muhammed bashir Khan" <muhammad.bashir.k...@univie.ac.at> To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, July 10, 2012 1:22:28 PM Subject: [ccp4bb] Crystal Optimization Dear All; Could somebody give a nice suggestion how the following type crystal could be optimized, I almost tried everything. Crystal Image is attached Crystal condition: 20% w/v PEG3350 and 200mM NaCl. Thanks in advance Bashir -- Muhammad Bashir Khan ************************************************** Structural Genome Consortium (SGC). University of Toronto Toronto, Canada
