If you need reducing agent, the best choice is probably TCEP. If you
are to choose between the BME and DTT, I'd recommend DTT, since BME
tends to modify cysteines (sometimes in active site which may be quite
annoying, although post-crystallization DTT soaks can remove some of
these). On the other hand, I know of at least one case whereby CYS-BME
modification has helped crystallization by mediating a crystal contact.
In summary, every protein is different and if you have capacity to try
BME, DTT and TCEP, try all three. And no reducing agent as well.
On 07/11/2012 04:50 AM, amro selem wrote:
Hallo every one,
i am working on one enzyme who has sulphur bridge , is using 2
mercaptoethnol or DTT during handling this protein for
crystallography is not desirable .
best regards
Amr
