Hi Bernhard -
Having the spots in regular lines indicates that this is a single
crystal diffraction pattern (to a first approximation). I have seen ice
rings which contain a few strong spots, probably from microcrystals of
ice, but I haven't yet seen ice diffraction that shows a lattice of
spots. So, the presence of the spot lattice, plus the spots below 3.9
A, allow one to say that this image isn't ice diffraction on top of
weak/absent protein diffraction. Ergo, this crystal is not a macromolecule.
Plus, I was trying to show Zhao that the spots aren't just scattered at
random.
- Matt
On 7/23/12 5:25 PM, Bernhard Rupp (Hofkristallrat a.D.) wrote:
> you'll see that some of them are arranged in regular lines.
I am not sure I understand what the line argument implies?
>indicates a very small unit cell, with dimensions probably < 10 A
Very indicative also the few strong and isolated high resolution
reflections
Cheers, BR
--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
