Dear All, re identifying metals,
It's possible to calculate crystallographic element-specific anomalous difference maps if you can collect data on either side of the absorption edge. I have used this successfully for Sr, Zn and Mn. http://www.ncbi.nlm.nih.gov/pubmed/15858259 I have a set of scripts to conveniently calculate such a map, which I can send off list. James -- Dr. James W. Murray David Phillips Research Fellow Division of Molecular Biosciences Imperial College, LONDON Tel: +44 (0)20 759 48895 ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Frank Murphy [frankvmur...@gmail.com] Sent: Wednesday, July 25, 2012 3:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] How to identify unknow heavy atom?? Dear Haytham, It should be relatively simple (and quick) to determine the metal in your sample employing either crystallized sample or solution using X-ray fluorescence (http://en.wikipedia.org/wiki/X-ray_fluorescence) at any beamline with a silicon drift detector (http://www.amptek.com/drift.html) set up for energy dispersive spectrometry (EDS). We perform these analyses regularly at NE-CAT and I am sure there are a number of other beamlines set up for the experiment. Frank Murphy Beamline Scientist NE-CAT / Cornell University frankvmur...@gmail.com
