XRF or TXRF would be very quick and definitive. With TXRF you could easily identify and quantify metals present in 1-10 uL of a 10 mg/mL solution. ICP-OES will also work, but will require about 300 ug or so of protein (depending on MW). ICP-MS is maybe 10x more sensitive than -OES but is more complex and exotic to use. We've used all these methods to characterize and quantify metalloenzymes.
Oxidation states may be revealed by visible absorption spectroscopy or inferred from coordination geometry and bond lengths. For some metal ions, EPR could be definitive for oxidation state. Atomic spectroscopy methods won't help here. Roger Rowlett On Jul 24, 2012 2:04 PM, "Theresa Hsu" <theresah...@live.com> wrote: > Does EXAFS requires same amount of samples as ICP-MS/ICP-AES? > > Theresa > > On Tue, 24 Jul 2012 12:55:31 -0500, Jacob Keller < > j-kell...@fsm.northwestern.edu> wrote: > > >Perhaps also exafs should be mentioned--I believe the various ion species, > >redox states, and even binding geometry can be determined. > > > >JPK > > > > > > > > > >-- > >******************************************* > >Jacob Pearson Keller > >Northwestern University > >Medical Scientist Training Program > >email: j-kell...@northwestern.edu > >******************************************* > > >
