Am 29 Jul 2012 um 18:53 hat Tatyana Sysoeva geschrieben: > > Hi! > > I heard a couple of times that use of cacodylate buffers in crystallization > is bad, and not only > because of the compound toxicity. > > As I understood, presence of the cacodylate in a protein crystal will cause a > particular crystal > degradation pattern upon X-ray exposure - "darkening of the crystals, gas > formation" > I tried to find some references on that and failed in doing so. > I found some earlier discussions like this one: > http://www.proteincrystallography.org/ccp4bb/message23691.html > but don't have anything to reference in literature. I would appreciate if > someone can point me to a > right direction. > > I am sorry if this question is out of the groups topic range. > > Thank you in advance! > Sincerely, > Tanya >
Dear Tanya, cacodylate can be attacked by nucleophilic side chains and can form covalent adducts, e.g.: http://www.jbc.org/content/278/3/2008.long Good luck, Karsten ------------------------------- Karsten Niefind University of Cologne Department of Chemistry Institute of Biochemistry Otto-Fischer-Str. 12-14 D-50674 Cologne Tel.: +49 221 470 6444 Fax: +49 221 470 3244
