Dear Tatyana,
We once had a project where the crystallization
condition contained cacodylate. The crystals diffracted
to 1.9A and survived reasonably well under the beam
(maybe there was indeed some colour change upon
exposure, I do not remember exactly).
We were working with drug soaks. The original structure is 1HYV.
The most interesting result of cacodylate presence
is modification of Cys residues. It was interpreted
as S-dimethylarsinoyl cysteine.
Best wishes,
Sergei
On 30-Jul-12 12:53 AM, Tatyana Sysoeva wrote:
Hi!
I heard a couple of times that use of cacodylate buffers in
crystallization is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal will
cause a particular crystal degradation pattern upon X-ray exposure -
"darkening of the crystals, gas formation"
I tried to find some references on that and failed in doing so.
I found some earlier discussions like this one:
http://www.proteincrystallography.org/ccp4bb/message23691.html
but don't have anything to reference in literature. I would appreciate
if someone can point me to a right direction.
I am sorry if this question is out of the groups topic range.
Thank you in advance!
Sincerely,
Tanya
--
Prof. Sergei V. Strelkov
Laboratory for Biocrystallography
Department of Pharmaceutical Sciences, Katholieke Universiteit Leuven
O&N2, Campus Gasthuisberg, Herestraat 49 bus 822, 3000 Leuven, Belgium
Work phone: +32 16 330845 Fax: +32 16 323469 OR +32 16 323460
Mobile: +32 486 294132
Lab pages: http://pharm.kuleuven.be/anafar
