The data sets were collected from the same crystal by "scan" collecting 40 frames from each section. The space group of this crystal is P2.
My guess that I may have to index and integrate each set indivadually, and then scale them together. Thanks Uma On Wed, Aug 1, 2012 at 9:01 AM, Machius, Mischa Christian < mischa_mach...@med.unc.edu> wrote: > Not much info to go by... > > Anyway, if the program 'goes crazy' you either have very different > exposure levels, radiation damage leading to non-isomorphism, or you have a > trigonal space group (P3xxx or P6xxx) and forgot to make sure all batches > are indexed the same way. > > If you have translated your crystal between batches, HKL2000 won't of > course be able to process all batches in one go. If you haven't touched the > crystals at all, and alln-in-one processing doesn't work, the parameters at > the end of one batch may not be accurate to start off a new batch, which is > mostly due to inaccurate goniostats. In that case, you will need to process > the batches individually and them combine them during scaling. > > Hope that helps. > > MM > > > On Aug 1, 2012, at 8:50 AM, Uma Ratu wrote: > > > Dear All: > > > > I collected 5 data sets from one crystal and would like to process them > together. > > > > Here is how I did: > > > > In HKL2000, load the all data sets. "Index" each set. When I try > "Intergrate", the program automatically go through the whole data sets > there, and do not go through. > > > > I then process data sets by loading one at each time. Index, intergrate > and scale all go through very smoothly. But when I put them together, the > program just goes crazy. > > > > Thank you for advice > > > > Uma > >
