Rajan, This is old news. Ron Kaplan published a lot about sarcosyl extraction of native mitochondrial carrier proteins from "inclusion bodies" in the late 1990's, but there is an equally robust literature stating that sarcosyl is a denaturant. It is very much dependent on a protein's characteristics as to whether it is sensitive to sarcosyl or not. One has to just try the experiment.
Cheers, Michael **************************************************************** R. Michael Garavito, Ph.D. Professor of Biochemistry & Molecular Biology 603 Wilson Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334 Email: rmgarav...@gmail.com **************************************************************** On Sep 20, 2012, at 10:33 AM, rajan kumar wrote: > here by i am sending you a paper it may help you. > > > regards > rajan > ACTREC > > <chaps and triton.pdf>
