Tim,
My understanding is that the D-isomer does not get incorporated. I could be wrong though. Bacteria may convert the D to the L-form. Chitta ----- Original Message ----- From: "Tim Gruene" <t...@shelx.uni-ac.gwdg.de> To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, November 12, 2012 4:25:34 AM Subject: Re: [ccp4bb] seleno methionine labelling of protein -----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear , I am not up to date, but is there an expression system that allows you to incorporate D-amino acids into proteins? In terms of phasing only the Se-atom is a point or sphere, so the signal won't be altered provided the position of that atom is the same in either case. Best, Tim On 11/12/2012 10:06 AM, Faisal Tarique wrote: > Dear All > > just want to ask the difference between labelling your protein with > D L selenomethionine mixture and L selenomethionine alone..will it > make difference in anomalous diffraction,detection of Se signal and > extraction of phases. Or will be the same for both..? > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFQoMCOUxlJ7aRr7hoRAopyAKD4kBksPb4zIDYDgok5r5BuuefndgCgrf3S r+YIxyjC50XywK1Tgc+fglk= =3GcK -----END PGP SIGNATURE-----
