Dear Yuri, I tried parathone oil once, and I can tell, that you need to be experienced in using it, so if you have some other crystals, you should get practice with them first. As for your crystal: shoot upon the matrix solvent first, and you will know is there are ice-rings or not . If yes, I would try to add glycerol to 5%, to shoot, still ice rings, then 10%. I think 10% should work. And then freeze your crystal. You may increase the glycerol concentration in 2 steps, 5% glycerol, then transfer the crystal to 10% glycerol. Good luck A.
-----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yuri Pompeu Sent: Friday, November 30, 2012 7:23 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] To cryo or not to cryo... Dear community, I have what seems to be a pretty decent single crystal that grew from a screen set up 2 weeks ago. I am trying to reproduce it but so far I have not succeeded. I am however afraid the crystal that did form will start to deteriorate. So this brings me to dilemma, I feel like I should try and mount this crystal and shoot it. But since I only have 1 sample, I do not want to mess this up... I am inclined to try cryo conditions, but I am afraid the addition of a cryo such as glycerol could destroy the little guy. The crystal formed in 30% PEG 4000, 0.1M NaCitrate pH5.6 and 0.2M NH4AcO, I wonder if this is a cryo condition already? Any suggestions would be appreciated. best,
