Dear All: Many thanks for your commoents and advice.
I will keep them in mind. Uma On Thu, Feb 14, 2013 at 7:54 AM, Fischmann, Thierry < thierry.fischm...@merck.com> wrote: > Collect small slices of data (instead of a complete data set) on several > crystals then merge the data together to get a full data set.**** > > The slices of must be small enough so that the damage to the S-NO group is > still very limited on each slice. You may have to play with beam > attenuation a bit, depending on how fast the degradation occurs.**** > > ** ** > > Good luck**** > > ** ** > > Thierry**** > > ** ** > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *Uma > Ratu > *Sent:* Wednesday, February 13, 2013 5:38 PM > > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] S-nitrosylation protein**** > > ** ** > > Dear All:**** > > **** > > I plan to use X-ray crystallography method to study the S-nitrosylated > protein structure. **** > > **** > > The native protein crystals diffracted to 2A with synchrontron. I now have > the crystals of S-ntrosylated protein. **** > > **** > > Since S-NO moiety appears to be unstable to synchrotron radiation, could > you advice / comments on the stratage on the data collection of > S-nitrosylated protein crystals? **** > > **** > > The protein crystals did not diffract well with in house X-ray. **** > > **** > > Thank you for your comments. **** > > **** > > Uma**** > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. >
