Hi Jacob, check out Figure 1 in
Natively inhibited Trypanosoma brucei cathepsin B structure determined by using an X-ray laser. Redecke L, et al. Science. 2013 Jan 11;339(6116):227-30. and In vivo protein crystallization opens new routes in structural biology. Koopmann R. Nature Methods. 2012 Jan 29;9(3):259-62. best Savvas On 15 Feb 2013, at 20:44, Jacob Keller wrote: > Dear Crystallographers, > > I was looking at some live, control HEK cells expressing just eGFP, and to my > great surprise, saw littered across the dish what appeared to be small > fluorescent needles (see attached--sorry about the size, but it's only ~1MB > total.) Can these possibly be fortuitous protein crystals? They were too > small to mount I think, and for what it's worth, parallel-transfected HeLa > cells did not have these things. But, some needles could be seen in the DIC > images as well, and the needles were only fluorescent with GFP filter sets, > and not CFP, YFP, or texas red filters. I thought of whale myoglobin > crystallizing on the decks of ships, but never thought I would see this.... > > Jacob > > -- > ******************************************* > Jacob Pearson Keller, PhD > Postdoctoral Associate > HHMI Janelia Farms Research Campus > email: j-kell...@northwestern.edu > ******************************************* > <GFP_crystals_DIC.png><GFP_crystals_Fluorescence.png>
