Dear All, thanks very much for the various suggestions--I finally made the connection. A good point was also made that gene synthesis is becoming ever cheaper (~$0.28/bp) so maybe PCRing from genomic DNA is not always advisable. I'll keep this in mind for the future, although for this one I had my reasons for going for the gDNA.
Thanks again, Jacob On Tue, Mar 5, 2013 at 1:07 PM, Jacob Keller <j-kell...@fsm.northwestern.edu > wrote: > Dear List, > > please pardon the major off-topicity, but I need a trace amount of V > cholera genomic DNA to PCR from, and it just kills me to spend ~$300 to > order it for this one and only use. Is anyone in the DC/Baltimore area > willing and able to donate this? I travel on the capitol beltway daily, so > anything nearby would work, esp. NIH. > > Jacob > > -- > ******************************************* > Jacob Pearson Keller, PhD > Postdoctoral Associate > HHMI Janelia Farms Research Campus > email: k <j-kell...@northwestern.edu>ell...@janelia.hhmi.org > ******************************************* > -- ******************************************* Jacob Pearson Keller, PhD Postdoctoral Associate HHMI Janelia Farms Research Campus email: k <j-kell...@northwestern.edu>ell...@janelia.hhmi.org *******************************************
