On Saturday, 16 March 2013, James Holton wrote: > The first report of shooting a protein crystal at a synchrotron (I > think) was in 1976: > http://www.pnas.org/content/73/1/128.full.pdf > that was rubredoxin > > The first PDB file that contains a "SYNCHROTRON=Y" entry is 1tld > (trypsin), which was deposited in 1989: > http://dx.doi.org/10.1016/0022-2836(89)90110-1 > But the structure of trypsin was arguably already "solved" at that time. > > Anomalous diffraction was first demonstrated by Coster, Knoll and Prins > in 1930 > http://dx.doi.org/10.1007/BF01339610 > this was 20 years before Bijvoet. But not with a synchrotron and > definitely not with a protein > > The first protein to be solved using anomalous was crambin in 1981: > http://dx.doi.org/10.1038/290107a0 > but this was not using a synchrotron > > The first demonstration of MAD on a protein at a synchrotron was a Tb > soak of parvalbumin in 1985 > http://dx.doi.org/10.1016/0014-5793(85)80207-6 > but one could argue that several parvalbumins were already known at that > time. > > The first MAD structure from native metals was cucumber blue copper > protein (2cbp) in 1989 > http://dx.doi.org/10.1126%2Fscience.3406739
The original CBP MAD structure (1CBP) was published in 1988. Also 1988: Lamprey hemoglobin (Fe MAD) DOI: 10.1002/prot.340040202 1989: Streptavidin (Se MAD): PNAS 1989 86 (7) 2190-2194 > The first "new" structure using MAD, as well as the first SeMet was > ribonuclease H (1rnh) in 1990 > http://dx.doi.org/10.1126/science.2169648 > > If anyone knows of earlier cases, I'd like to hear about it! Ethan > > -James Holton > MAD Scientist > > On 3/13/2013 7:38 AM, Alan Cheung wrote: > > Hi all - i'm sure this many will know this : when and what was the > > first protein structure solved on a synchrotron? > > > > Thanks in advance > > Alan > > > > >
