Hi Randy and Rodger, We have had real success using BlueSky (MA) for insect expression, I believe we have had around 15-20 genes synthesis-optimized with these guys. The expression levels have been rather reasonable too (obviously protein dependent, but on average 1 mg of xtal grad protein per 1g of cell pellet). The largest construct we did I believe was around 90kDa.
Cheers, Mat ________________________________ From: Roger Pickman <rpick...@googlemail.com> To: CCP4BB@JISCMAIL.AC.UK Sent: Thursday, 9 May 2013, 10:31 Subject: Re: [ccp4bb] Codon Optimization for Insect Expression I hope this isn't too much of a thread hijack, but i also wanted to add a question to Randy's : How big a protein can one express in insect cells? I've read in a few places that it's not size limited but i wonder if that's borne out in practice. On 9 May 2013 16:05, Randy Watson <sigepra...@gmail.com> wrote: Hi all, >Sorry for the off-topic request. I am considering ordering codon-optimized genes for expression in insect cells (High Five). I wonder if anyone could make suggestions regarding: which companies are best to order from (accurate synthesis – cost efficiency) and what optimized codon sequence generators yield the best protein expression (as I understand it, not all algorithms are created equal). >Thanks, >Randy
