You can always synthesize your own too! Artem
Or idtdna. Dna2.0, blue sky, and many others On May 9, 2013 10:01 AM, "Mathew Martin" <mathew.mar...@rocketmail.com> wrote: > Hi Randy and Rodger, > > We have had real success using BlueSky (MA) for insect expression, I > believe we have had around 15-20 genes synthesis-optimized with these guys. > The expression levels have been rather reasonable too > (obviously protein dependent, but on average 1 mg of xtal grad protein per > 1g of cell pellet). The largest construct we did I believe was around 90kDa. > > Cheers, > > Mat > > ------------------------------ > *From:* Roger Pickman <rpick...@googlemail.com> > *To:* CCP4BB@JISCMAIL.AC.UK > *Sent:* Thursday, 9 May 2013, 10:31 > *Subject:* Re: [ccp4bb] Codon Optimization for Insect Expression > > I hope this isn't too much of a thread hijack, but i also wanted to add a > question to Randy's : How big a protein can one express in insect cells? > I've read in a few places that it's not size limited but i wonder if > that's borne out in practice. > > > > On 9 May 2013 16:05, Randy Watson <sigepra...@gmail.com> wrote: > > Hi all, > Sorry for the off-topic request. I am considering ordering > codon-optimized genes for expression in insect cells (High Five). I wonder > if anyone could make suggestions regarding: which companies are best to > order from (accurate synthesis – cost efficiency) and what optimized codon > sequence generators yield the best protein expression (as I understand it, > not all algorithms are created equal). > Thanks, > Randy > > > > >
