I keep sending mails by accident today - apologies for the spam. The
last sentence of my should read:
This could of course be due to too high a concentration of mother
liquor but quite often it occurs at relative humidity values where the
concentration of the mother liquor components will not have increased by
very much. Cheers, Matt.
On 2013-05-23 15:32, Ed Pozharski wrote:
Matt,
with this technique, how do you prevent crystal from drying up (other
than "doing it fast")? I know Thorne's group does this trick under
oil.
If you take no extra precautions, do you have an estimate of how
often
diffraction is destroyed by this?
On the other hand, it's quite possible that what destroys resolution
when crystals dry up is increase in concentration of non-volatile
mother
liquor components, which shouldn't be happening here to the same
degree.
Cheers,
Ed.
On Thu, 2013-05-23 at 14:38 +0200, Matthew BOWLER wrote:
Hi Faisal,
if your solvent channels are smaller than 40A in the largest
dimension
(most are) you can use a mesh loop to pick up the crystal and then
wick
away all of the mother liquor. You can then flash cool your crystal
without having to transfer the crystal to another solution. Good
luck,
Matt
--
Matthew Bowler
Synchrotron Science Group
European Molecular Biology Laboratory
BP 181, 6 rue Jules Horowitz
38042 Grenoble Cedex 9
France
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