Glenn Masson,
We had crystals that appeared by chance at "underfined" low temparature:
Maïga, A., Vera, L., Marchetti, C., Lorphelin, A., Bellanger, L., Mourier,
G., Servent, D., Gilles, N. & Stura, E. A. (2013) Crystallization of
recombinant Green mamba ρ-Da1a toxin during lyophilization procedure and
its structure determination. Acta Cryst. F 704-709.
There is an intereasting zone for crystallization around zero, but the
problem is going to be how to observe the crystals and
accurately control the temperature.
Enrico.
On Thu, 30 May 2013 13:26:44 +0200, Glenn Masson <glennmas...@gmail.com>
wrote:
Hello CCP4BBers,
I am currently playing with some crystals that seem to enjoy lower
temperatures, and I was thinking of breaking the 0°C threshold.
Looking for examples of this in the literature is problematic, as
searching
for examples in the PDB (Under the advanced search-> crystal properties->
temperature (K)) turns up a large amount of false positives. Many
otherwise
supremely intelligent people seem unable or unwilling to grasp the
concept
of Kelvin (it's amazing how many protein structures were solved only 22
degrees above absolute zero...).
I was wondering if anyone has much experience in this area? I see a few
structures e.g. 2Z97 (-5°C) and 4H0W (-2°C), but I was wondering if
anyone
has a more systematic knowledge, some more examples, and what the
parameters and best practice of this technique are.
Many thanks,
Glenn Masson>
MRC-Laboratory of Molecular Biology
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
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http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
