If one has cryoprotectant in their conditions, one may be surprised that 
crystals can grow at -20 deg C and probably lower.  Practice with lysosyme. :)

________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Glenn Masson 
[glennmas...@gmail.com]
Sent: Thursday, May 30, 2013 6:26 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystallisation below 0°C

Hello CCP4BBers,

I am currently playing with some crystals that seem to enjoy lower 
temperatures, and I was thinking of breaking the 0°C threshold.

...

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