Maybe I was a bit hasty- the Hb example gives only 2.4% difference;
2.0 at CuKa, if I'm interpreting the output correctly.
Maybe in the F. transform the random noise averages out and the signal
rises above.
But the Hb xtal probably diffracts really well with R-merge 3 or 4 in
the low resolution shell. This is a huge multisubunit membrane protein
complex, such as I'm happy with overall rmerge 12% and 6-8% in the low
resolution shell. But if I don't say that in the grant proposal . . .
Anyway this is all hypothetical now- the protein still needs to be expressed,
purified, crystallized.
Thanks,
eab
Jim Pflugrath wrote:
How does that compare to something that readily works with Fe, such as horse
hemoglobin on a home lab copper X-ray system with 2 Fe in 291 residues in the
asymmetric unit?
________________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Edward A. Berry
[ber...@upstate.edu]
Sent: Saturday, June 01, 2013 6:46 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] anomalous scattering server down?
That's what I wanted. Results are not promising- Bijvoet differences
would be less than 2% at peak, and this is not lysozyme.
Thanks, all,
eab
Mooers, Blaine H.M. (HSC) wrote:
Bernhard Rupp has a calculator of Bijvoet ratios:
http://www.ruppweb.org/new_comp/anomalous_scattering.htm
Blaine Mooers
Assistant Professor
Director Macromolecular Crystallography Lab
Member Stephenson Cancer Center
Department of Biochemistry and Molecular Biology
University of Oklahoma Health Sciences Center
S.L. Young Biomedical Research Center Rm. 466
Letter address:
P.O. Box 26901, BRC 466
Oklahoma City, OK 73190
Shipping address:
975 NE 10th Street, BRC 466
Oklahoma City, OK 73104-5419
office: (405) 271-8300 lab: (405) 271-8313 fax: (405) 271-3910
e-mail: blaine-moo...@ouhsc.edu
Faculty webpage:
http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/faculty/blaine-mooers-ph-d-
X-ray lab webpage:
http://www.oumedicine.com/department-of-biochemistry-and-molecular-biology/department-facilities/macromolecular-crystallography-laboratory
SAXS Links webpage:
http://www.oumedicine.com/docs/default-source/ad-biochemistry-workfiles/small-angle-x-ray-scattering-links.html?sfvrsn=0
________________________________________
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Edward A. Berry
[ber...@upstate.edu]
Sent: Saturday, June 01, 2013 6:09 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] anomalous scattering server down?
Hmm- looks like crosssec calculates F' and F" (and cros-section).
I guess you're thinking of the "x-ray edge" page on the same server.
Ive got local copies and graphs of that in an excel spreadsheet.
I was thinking about the page where you enter your protein's
molecular weight, number and type of anomalous scatterers, and
it tells you how accurate your data has to be to make the
anomalous signal significant.
Thanks,
eab
Bosch, Juergen wrote:
seems to be down sorry.
you should be able to use crossec from ccp4
Jürgen
On Jun 1, 2013, at 6:47 PM, Edward A. Berry wrote:
Is Ethan Merritt's anomalous scattering page at:
https://urldefense.proofpoint.com/v1/url?u=http://www.bmsc.washington.edu/scatter/&k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0A&r=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0A&m=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0A&s=57fb37f57cec12f6c778c53c6bfbdd04afc795f050605681dca25305998c6af0
down or moved, or the firewall I'm behind is blocking it?
I want to check feasibility of a native-iron MAD experiment,
and I'm not very good at math.
thanks,
eab
......................
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Office: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-2926
https://urldefense.proofpoint.com/v1/url?u=http://lupo.jhsph.edu/&k=7DHVT22D9IhC0F3WohFMBA%3D%3D%0A&r=ftLbjJYpc5s5JQz9Q6qd7uT7FxPLb4V0aIwH4RJhyZU%3D%0A&m=vriAf%2FYLAE3OBEruPk8rCrKni5F1ZSfZwaGrrX0lwuk%3D%0A&s=d80576bcb5bffce1fe3f7ffe04c9ec7a4b3338c452e984d4efcbd8036ce5b83c
