Hi Theresa, I think you have to be very careful with NMR of homo-oligomers, even if they’re small proteins: the NMR model/structure (backbone only) of a small integral membrane kinase was a huge effort - http://www.ncbi.nlm.nih.gov/pubmed/19556511
but is very different from the recently published crystal structure - http://www.ncbi.nlm.nih.gov/pubmed/23676677 There’s some commentary here: http://www.nature.com/nature/journal/v497/n7450/full/nature12245.html I’m fairly biased but i don’t think that both states could exist – and going from the NMR model to the crystal structure would require a huge amount of energy – a morph between the two can be found here: http://youtu.be/5vpEGSvVe04 Needless to say we couldn’t (try as we might!) get an MR solution using the NMR model/structure.... Best wishes, val On 9 June 2013 16:36, Theresa Hsu <theresah...@live.com> wrote: > Dear all > > A question for the cross-trained members of this forum - for small sized > proteins, is NMR better than crystallography in terms of data collection > (having crystals in the first place) and data processing? How about > membrane proteins? > > I would appreciate replies to the board, instead of off-board, to allow > for a good discussion. > > Thank you. > > Theresa >
