On Wed, 24 Jul 2013 16:36:17 +0200, Hena Dutta <hdutt...@gmail.com> wrote:
Hi,
Can anyone tell, if there is any database containing the crystallization
conditions of published structures? I want to see the conditions people
have used for those proteins having some structural similarity. Any
suggestion would be appreciated.
Regards...
Hena
You can use the reports in the PDB database:
1) Sequence search with your sequence
2) Report pull down menu -> select Crystallization
How is that going to help? Even a single amino acid change can change the
manner a protein
crystallizes! The precipitant concentration is affected by the protein
concentration.
The purity and purification procedure can also affect crystallization.
Enrico.
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
http://www-dsv.cea.fr/ibitecs/simopro/ltmb/cristallogenese
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://scholar.google.com/citations?hl=en&user=Kvm06WIoPAsC&pagesize=100&sortby=pubdate
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
