Dear all, I was about to deposit a few structures to the pdb when I noticed the mean B-factors were larger than one might expect.
All the structures were refined using TLS refinement. During refinement in Refmac the average temperature factors for each structure is reasonable. For example, a structure at 2.75Å has a mean B-factor of 40; however, after adding the ANISOU records as required by the PDB, I noticed the average B-factors double. Is this normal? Sincerely, Omid --------------------------------------------------- --------------------------------------------------- Omid Haji-Ghassemi, Graduate Student Department of Biochemistry & Microbiology University of Victoria PO Box 3055 STN CSC Victoria, BC, V8W 3P6 CANADA Tel: 250-721-8945 Fax: 250-721-8855
