Hi Fellows,
could someone please point me towards the source of a known high purity imidazole with low absorbance at 280 nm? I am facing the problem of detecting a low absorption protein in high imidazole background after IMAC gradient elution. In the UV spectra of the 2 imidazoles I checked there is some contaminant that absorbs at 280 Thx, BR ---------------------------------------------------------------------------- ------------ Bernhard Rupp Marie Curie Incoming International Fellow Innsbruck Medical University Schöpfstrasse 41 A 6020 Innsbruck Austria +43 (676) 571-0536 <mailto:bernhard.r...@i-med.ac.at> bernhard.r...@i-med.ac.at ---------------------------------------------------------------------------- ------------ Dept. of Forensic Crystallography k.-k. Hofkristallamt Vista, CA 92084 001 (925) 209-7429 <mailto:b...@ruppweb.org> b...@ruppweb.org <mailto:b...@hofkristallamt.org> b...@hofkristallamt.org <http://www.ruppweb.org/> http://www.ruppweb.org/ -----------------------------------------------------------------------
