Would you happen to know what the A280 of a 1M (or .1 M) solution is?
I wonder if this absorbance is really due to impurities or is the tail of a weak absorbance band of imidazole itself. I
notice A280 is not one of the properties sigma lists for its imidazole.
Jan wrote:
Hi Bernhard,
this one works for us:
Sigma BioUltra imidazole, >99.5% by GC, 56749
Cheers,
Jan
--
Jan Abendroth
Emerald BioStructures
Seattle / Bainbridge Island WA, USA
home: Jan.Abendroth_at_gmail.com
work: JAbendroth_at_embios.com
http://www.emeraldbiostructures.com
On Aug 21, 2013, at 7:33 AM, Bernhard Rupp <hofkristall...@gmail.com
<mailto:hofkristall...@gmail.com>> wrote:
Hi Fellows,
could someone please point me towards the source of a known high purity
imidazole
with low absorbance at 280 nm? I am facing the problem of detecting a low
absorption protein
in high imidazole background after IMAC gradient elution. In the UV spectra of
the
2 imidazoles I checked there is some contaminant that absorbs at 280…
Thx, BR
----------------------------------------------------------------------------------------
Bernhard Rupp
Marie Curie Incoming International Fellow
Innsbruck Medical University
Schöpfstrasse 41
A 6020 Innsbruck – Austria
+43 (676) 571-0536
bernhard.r...@i-med.ac.at <mailto:bernhard.r...@i-med.ac.at>
----------------------------------------------------------------------------------------
Dept. of Forensic Crystallography
k.-k. Hofkristallamt
Vista, CA 92084
001 (925) 209-7429
b...@ruppweb.org <mailto:b...@ruppweb.org>
b...@hofkristallamt.org <mailto:b...@hofkristallamt.org>
http://www.ruppweb.org/
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