What does a straight difference map look like? i.e. omit one nucleotide at a time, do a few cycles of refinement and then inspect the weighted difference map - SA may be too violent for your structure. Eleanor On 4 Nov 2013, at 06:36, dengzq1987 wrote:
> Dear all, > > Recently, I received the comments from referees, they asked for the SA-omit > map of the ssDNA of our protein-DNA complex. They said that simulated > annealing omit map better than a biased 2Fo-Fc. The ssDNA consists of seven > thymidine nucleotide. Our data diffracted to 2.65A，but the data quality is > not good and twin. We tried to produce SA-omit map using phenix. The map is > really bad. Does anyone have suggestion to refine the map? Thank you! > > > Bests, > zq Deng > 2013-11-04 > dengzq1987
