-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear zq Deng,
you may actually have some anomalous signal from the P-atoms in your structure, even if you collected at short wavelength - if you have a data set collected at, say, 1.5A, it would be even stronger. In that case you could create an anomalous difference map (you need unmerged data). If that show peaks near the P-atoms, you have pretty model-bias confirmation for the position of your DNA. George Sheldrick's program anode is very convenient to use for such purpose, but you need unmerged Bijvoet-pairs in your data, of course. Best, Tim On 11/04/2013 07:36 AM, dengzq1987 wrote: > Dear all, > > > > Recently, I received the comments from referees, they asked for > the SA-omit map of the ssDNA of our protein-DNA complex. They said > that simulated annealing omit map better than a biased 2Fo-Fc. The > ssDNA consists of seven thymidine nucleotide. Our data diffracted > to 2.65A?but the data quality is not good and twin. We tried to > produce SA-omit map using phenix. The map is really bad. Does > anyone have suggestion to refine the map? Thank you! > > > > > > Bests, > > zq Deng > > 2013-11-04 > ------------------------------------------------------------------------ > > dengzq1987 - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.15 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iD8DBQFSd5pdUxlJ7aRr7hoRAvm8AKCMpTi6+U8pmcv9kTonB+7Rs9SQwQCff4Vg 0IedSwdjUXW6PIMwTMnURpY= =1RSo -----END PGP SIGNATURE-----
