Good Morning to ALL,

I encountered large gaps between R-factor and R-free, eg. 10% and 11%, during refining several structures with either Refmac or Phenix.refine. The data were reduced and scaled at resolution with I/SIGMA higher than 2.3. The model fits and density pretty well despite a few side chains missing density.

I may ask for the reasons that result in such large difference between R and R free and some ways to examine and improve the refinement.

 Thanks in advance

Wenhua

Univ. Paris-Sud XI, Orsay


On 11/25/2013 6:03 PM, Beatrice Vallone wrote:
Please check the announcement for 2014 ESRF Users' Meeting & Associated Structural Biology Workshop.

Invitation to the 2014 ESRF Users' Meeting & Associated Workshops to meet and discuss with fellow users, get inspired, establish new scientific collaborations and learn about future opportunities offered by the ESRF for your research.

Workshop on*"Structural Biology at ESRF: Past, Present & Future <http://www.esrf.eu/home/events/conferences/um2014/structural-biology-workshops.html>"*
3 - 5 February

The Plenary Meeting  will feature:

  *    5 plenary lectures by world leading scientists covering a large
    spectrum of ESRF activities;
  * a presentation on the status of the facility and on the progress
    of the Upgrade Programme;
  * a talk by the winner of the prestigious "ESRF Young Scientist Award";
  * a poster session;
  * a banquet, with awards to the ESRF Young Scientist Award and for
    the best poster

Programs for the Meeting and Workshops are available at:

http://www.esrf.eu/home/events/conferences/um2014.html

   Regards to all,

   Beatrice Vallone
ESRF User Organization Committee




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