check an anomalous map!
The obvious thing to do to rule out gold binding
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Dr. Matthias Zebisch
Division of Structural Biology,
Wellcome Trust Centre for Human Genetics,
University of Oxford,
Roosevelt Drive,
Oxford OX3 7BN, UK
Phone (+44) 1865 287549;
Fax (+44) 1865 287547
Email matth...@strubi.ox.ac.uk
Website http://www.strubi.ox.ac.uk
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On 12/10/2013 12:44 PM, PriyankMaindola wrote:
dear members
i am trying to solve this crystal structure but
I am puzzled with an
undefined blob
that
appeared at a glutamine residue after refinement. I have attached
pics of that below.
Is it a covalent modification of acid-amide side chain..
... as there is no charged environment around and density seems
continuous.
please suggest
....
following reagents were encountered by protein
during purification, crystallization and soaking
:
phenyl methyl sulfonyl fluoride
benzamidine
tris
dtt
(could it be cyclized dtt?)
k[au(cn)2]
acidic pH
isopropanol
citrate
sulfate
phosfate
K+, Na+, Cl-
map contour:
2fo-fc: 1rmsd
fo-fc (green): 3 rmsd
--
*Priyank*
