Ho Jun Lee, Have you thought about differential scanning fluorimetry (Thermofluor)? With this biophysical technique you can characterize protein-ligand interactions and screen a wide variety of ligands using minimal concentration of ligand and protein. All you need is a quantitative PCR machine (qPCR). Here is a reference for you https://chemistry.osu.edu/~magliery/pdfs/LavinderMagliery2009JACS.pdf
Lorenzo Ihsan Finci, Ph.D.Postdoctoral Scientist, Wang LaboratoryHarvard Medical SchoolDana-Farber Cancer InstituteBoston, MA Peking UniversityCollege of Life SciencesBeijing, China Date: Mon, 13 Jan 2014 21:50:07 +0900 From: hojunle...@gmail.com Subject: [ccp4bb] Assays for protein-ligand interaction? To: CCP4BB@JISCMAIL.AC.UK Sorry for the off topic. I'm looking for a way to monitor protein-(potential) ligand interaction. The ligand is small molecule (mw~250) and we're looking for its potential interaction with couple human proteins. (We do not know this small molecule interacts with these human protein or not.) Is there any efficient way to quickly identify whether this ligand interacts with those human protein? We can buy some protein, but the amount of commercially available purified proteins is very little, making them hard to be analyzed by some good methods (e.g. ITC). That would be really great if anyone suggest any idea. Sorry for the off topic question again. Thanks!
