Specifically for fluorescence does your ligand fluoresce?
It is possible if it has indol group or some aromatic organic compound Does your protein has a tryptophan or tyrosines in the binding site? If yes may be a fluorescence titration experiment could be the solution. Also fluorescence needs very low concentration of protein (nM to microM) george From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Acoot Brett Sent: Monday, January 13, 2014 3:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Assays for protein-ligand interaction? FRET， CD， Fluorescence， NMR chemical shift assay, isotope-labelled ligand interaction assay, protein melting temperature assay, gel filtration retention assay, gyration radius assay by Malls, native page gel analysis, etc. Acoot On Monday, 13 January 2014 8:51 PM, HJ Lee < <mailto:hojunle...@gmail.com> hojunle...@gmail.com> wrote: Sorry for the off topic. I'm looking for a way to monitor protein-(potential) ligand interaction. The ligand is small molecule (mw~250) and we're looking for its potential interaction with couple human proteins. (We do not know this small molecule interacts with these human protein or not.) Is there any efficient way to quickly identify whether this ligand interacts with those human protein? We can buy some protein, but the amount of commercially available purified proteins is very little, making them hard to be analyzed by some good methods (e.g. ITC). That would be really great if anyone suggest any idea. Sorry for the off topic question again. Thanks!
