Wasn't there this huge thread just 3 days ago on heavy atom soaking????
On 19/01/2014 07:18, Felix Frolow wrote:
Francis, It can happened
We have (not yet published) P1 with 24 molecules. When we cut His-tag we get
P1 with 32 molecules.
In our case we believe it is dictated by very strong interaction between two
monomers, and strong interaction between dimers with build a flattish tetramer.
Probably such formations
is more difficult to oaks than globular oligomers.
In this moment I do not recall what we see in solution, I have to check.
Relating to structure solution, P1 is very convenient space group.
I would go for determination this structure by SAD (SHELXC/D/E pipe, PHENIX or
SHARP). For the native - molecular replacement.
In our time after tremendous developments in Refmac and Phenix and development
o DM refinement is 3-3.4 Ang. Is not very difficult.
I would use in addition to NCS restraints in refinement also multi crystal
averaging. Roumors say it is the most strongest phasing method (attributed to
Eleanor Dodson, myself never used it).
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and Biotechnology
Tel Aviv University 69978, Israel
Acta Crystallographica F, co-editor
e-mail: mbfro...@post.tau.ac.il
Tel: ++972-3640-8723
Fax: ++972-3640-9407
Cellular: 0547 459 608
On Jan 19, 2014, at 08:48 , Francis Reyes <francis.re...@colorado.edu> wrote:
You sure about this space group? 24 monomers in P1 is unusual (at least to me)
F
On Jan 18, 2014, at 9:14 AM, Chris Fage <cdf...@gmail.com> wrote:
Hello Everyone,
I am currently trying to phase a structure with an asymmetric unit predicted to
contain 20-24 monomers (space group P1). The native crystals, while beautiful
in appearance (see attached), only diffract to ~3.4-3.0 angstroms at best, and
SeMet-derived crystals grow with poor morphology (small needles). Also, based a
fluorescence scan, I know that mercury does not bind appreciably. Other than
screening for a new space group, what options might I have for phasing this
many monomers at lower resolution? Is there any real chance of solving the
structure in this space group?
Thank you in advance for any suggestions!
Regards,
Chris
<Crystals.jpg>
