Dear Colleagues, Apologies for the off-topic question:
We are developing an approach for ‘Protein Repair’ to be utilized for the enhancement of activity defective proteins. Ideal candidates for us are enzymes that can be isolated for in-vitro activity assays, and for which single amino acid substitution/s are known to reduce their activity to ~ 20-80% of the wt. Typically, this would correspond to mutations causing a mild loss of instability and/or allosteric interference, but NOT to active-site mutations resulting in completely dead enzymes. If you are aware of such examples, we would be extremely happy to hear about it. Thank you in advance for any suggestions. Regards, Hay ======================================== Hay Dvir Ph. D. Head Technion Center for Structural Biology Technion Haifa 3200003, Israel Tel: +(972)-77-887-1901 Fax: +(972)-77-887-1935 E-mail hd...@technion.ac.il Website http://tcsb.technion.ac.il/Hay-Dvir ========================================
