Dear Mark Thank you for yor reply, and yes I have tried adding it to the maltose resin after cleavage but the MBP runs through with my protein, I have also tried 1M NaCl but with no luck and I also apply detergent after cleavage to the dialysis buffer because I usually dialyze after cleavage , is there anything that could maybe precipitate the MBP Best Regards Rana
________________________________ From: Mark J van Raaij <mjvanra...@cnb.csic.es> To: rana ibd <rna19792...@yahoo.com> Sent: Thursday, March 27, 2014 11:35 AM Subject: Re: [ccp4bb] maltose binding protein did you try the maltose-resin? in principle it should bind MPB but not your protein. You can try to add salt or detergents to disturb interaction between MBP and your protein (also in gel filtration). No guarantee of success, unfortunately not all protein behave "nicely". Mark J van Raaij Lab 20B Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 27 Mar 2014, at 11:26, rana ibd wrote: > Dear CCP4 > Does anyone know how to remove the maltose binding protein after cleavage > from the target protein; I have tried gel filtration and ion exchange but > with no luck, my protein is interacting with the MBP even after complete > cleavage. I would be grateful for any help or suggestions > Best Regards > Rana
